Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신성찬 | - |
dc.contributor.other | Jin-Sik Choi | - |
dc.contributor.other | Byung-Joo Lee | - |
dc.contributor.other | Hee-Young Park | - |
dc.contributor.other | Ji-Sun Song | - |
dc.contributor.other | Sung-Chan Shin | - |
dc.contributor.other | Jin-Choon Lee | - |
dc.contributor.other | Soo-Geun Wang | - |
dc.contributor.other | Jin Sup Jung | - |
dc.date.accessioned | 2016-04-19T11:18:41Z | - |
dc.date.available | 2016-04-19T11:18:41Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.other | 2015-OAK_2015_SCI_신성찬_1 | - |
dc.identifier.uri | http://repository.pnuh.or.kr/handle/2015.OAK/406 | - |
dc.description.abstract | OBJECTIVES: Human mesenchymal stem cells (MSCs) are efficacious in various cellular therapeutic applications and have been isolated from several tissues. Recent studies have reported that human tonsil tissue contains a new source of progenitor cells, potentially applicable for cell-based therapies. Information about the effects of donor age, long-term passage and cryopreservation are essential for clinical applications and cell-based therapies. Therefore, the authors investigated how the morphology, cell-surface markers, proliferation potential and differentiation capacity of tonsil-derived MSCs (T-MSCs) were affected by donor age, long-term passage, and cryopreservation. MATERIALS AND METHODS: T-MSCs were isolated from tonsillar tissue of 20 patients undergoing tonsillectomy. Authors evaluated the effects of donor-age, long-term passage, and cryopreservation on the morphology, surface markers, proliferation potential and differentiation capacities of T-MSCs. RESULTS: T-MSCs exhibited a fibroblast-like, spindle-shaped appearance. There were no significant morphological differences according to donor age, long-term passage or cryopreservation. T-MSCs isolated from donors of various ages were positive for markers CD90, CD44, and CD73, but negative for CD45, CD31, and HLA-DR. There were no significant differences in the expression of positive and negative surface markers as a function of donor age, long-term passage and cryopreservation. T-MSCs from different donor age groups showed similar proliferation potentials after passage 2. After long-term passage and cryopreservation, there were no significant morphological differences. Cryopreservation did not affect the proliferation potential of T-MSCs, but there was a significant decrease in the proliferation potential in long-term passage T-MSCs (passage 15). The effect of donor age, long-term passage and cryopreservation on the in vitro adipogenic, osteogenic, and chondrogenic differentiation potential of T-MSCs was not significant. CONCLUSION: The effect of donor age, long-term passage culture, and cryopreservation on T-MSC properties are negligible, except for the proliferation capacity of long-term cultured T-MSCs. Therefore, T-MSCs are considered to be promising MSCs that can be used as future alternative sources for autologous or allogenic MSCs | - |
dc.format.extent | - | |
dc.title | Effects of Donor Age, Long-Term Passage Culture, and Cryopreservation on Tonsil-Derived Mesenchymal Stem Cells | - |
dc.type | Thesis | - |
dc.author.department | 이비인후과 | - |
dc.author.google | Shin, Sung Chan | - |
dc.relation.issue | 36(1) | - |
dc.relation.volume | 85-99 | - |
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